IQ Webinar: Dual optical-electrical nanopore sensor to enable quality control in biomanufacturing
June 12, 2024
Webinar
Location: virtual
Venue: Webex Webinar
IQ Webinar: Dual optical-electrical nanopore sensor to enable quality control in biomanufacturing
Wednesday, 12 June 2024
11 AM - 12 PM ET
Presented by the Nanotechnology and Nanomaterial Working Group
Sponsored by the IQ Drug Product Leadership Group
SPEAKERS/MODERATOR
- George Alexandrakis, PhD (Univ. of Texas at Arlington)
- Jeffrey D. Clogston, PhD (Nanotechnology Characterization Lab)
- Patrick Lim Soo, PhD (Pfizer, Inc.)
Alternating current (AC) modulation of the voltage applied across a nanopore that is capped by a thin layer of gold enables acquisition of new data types that enhance the characterization of both targeted proteins and gene delivery nanoparticles for improved quality control during their production. This sensor provides label-free optical-electrical measurements at the single protein and nanoparticle levels. For the case of nanoparticles carrying a gene load (LNPs, AAVs) the sensor traps individual particles optically and then measures their impedance over a broad range of electrical current frequencies. Lower electrical current frequencies travel around each particle (testing the integrity of its coating), while higher frequencies travel through the particle (probing directly its DNA/RNA load). As a result, these the sensor measurements will quantify the fraction of empty versus filled particles and possibly even detect partially filled ones. In the case of protein-protein interaction analysis, the strength of this sensor is in its ability to quantify interactions at ultralow interactions, down to the atto-molar level, by concentrating analytes into a nanoscopic volume over the sensor's optical trap. Measurements of protein-protein interactions over a broad range of electrical current frequencies enables superior separation between specific versus non-specific interactions. In addition, AC sensing measurements make analysis of both protein and nanoparticle solutions more robust to impurities compared to currently available methods. In all, the novel abilities of this sensor technology can potentially make it useful during the early steps of biomanufacturing processes before costly resources are committed to the subsequent scale-up step.
This webinar is open to the public. IQ Membership is not required. (View member list)